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Rationally Designed Aquatic Receptors integrated in label-free biosensor platforms for remote surveillance of toxins and pollutants (RADAR)
Start date: Jan 1, 2011, End date: Dec 31, 2014 PROJECT  FINISHED 

RADAR is a 7-member consortium that aims to develop a robust, sensitive, and versatile label-free, biosensor platform for spot measurements and on-line monitoring of toxins and pollutants in food production processes and in the aquatic environment.Specificity towards chemical pollutants and toxins is achieved by using recombinant receptors (namely the estrogen receptor and the aryl hydrocarbon receptor) whose amino acid sequences have been rationally designed based on genomic and functional information from aquatic organisms.Sensitivity of the biosensor is increased by the unique combination of isotachophoretic pre-concentration step, and surface nanostructuring & chemical modification.The integration of the label-free detection sensors with an on-line automated sample handling and a wireless communication system will yield a best-in-class biosensor platform for robust, specific and sensitive detection of EDCs and PAHs in difficult operating conditions.To validate the RADAR biosensor the consortium will test the biosensors in fresh and marine water, in fish farms, and in food products such as fish, fruit juices, and milk. Through their contacts in these industries, the partners will evaluate the performance of the biosensors in such environments, analyzing a representative number of samples and reporting on the stability, ruggedness and accuracy of the sensors used under laboratory and real test conditions.This project is expected to have a high economic impact, since our cost-effective sensor could find a worldwide distribution in most food production and water testing lines as supported by Agilent Technologies Inc.

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