On-chip simultaneous intracellular recording and s.. (BRAIN STORM)
On-chip simultaneous intracellular recording and stimulation of electrical and biochemical activities from hundreds of neurons
Start date: 01 Jan 2008,
End date: 31 Dec 2010
In the BRAINSTORM project, we aim to develop an integrated system enabling on-chip intracellular recordings from a relatively large cultured neuronal network combined with chemical sensing and intracellular delivery of biologically active molecules. To achieve tight coupling with the cell membrane and enable efficient recordings, micronail-type probes will be integrated with a CMOS readout circuitry. These micronails are structured to operate either as intracellular microelectrodes or as intracellular micro-syringes allowing either chemical injection or to produce pressure or suction that will be used in additional to other procedures to penetrate the cell membrane. With appropriate bio-functionalization, the micro-nails inserted intracellularly will also serve as sensors for phosphorylation/ dephosphorylation reactions within living cells.The main technologies and methods to be developed in the BRAINSTORM project include:\tBiocompatible and CMOS compatible micronails and micro-syringes with integrated readout electronics;\tMicrofluidic system integrated with the CMOS-containing chip;\tProcedures to insert both nail types through the plasma membrane, into the neuronal cytosole;\tSurface chemistries allowing to achieve a Giga-Ohm (G) seal resistance between the disrupted plasma membrane and the nail;\tHigh signal-to-noise ratio recordings of neuronal electrical activity with micronail probes;\tOn-chip chemical delivery of chemicals;\tOn-chip sensing of intracellular kinase activities.
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